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Merck
모든 사진(1)

문서

73144

Sigma-Aldrich

Resorufin

95%

동의어(들):

7-Hydroxy-3H-phenoxazin-3-one, NSC 12097

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About This Item

실험식(Hill 표기법):
C12H7NO3
CAS Number:
Molecular Weight:
213.19
Beilstein:
174850
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.32

Quality Level

분석

≥95.0% (HPLC)
95%

형태

solid

mp

>300 °C (lit.)

저장 온도

−20°C

SMILES string

O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2

InChI

1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H

InChI key

HSSLDCABUXLXKM-UHFFFAOYSA-N

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관련 카테고리

일반 설명

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.
Zhou M, et al.
Analytical Biochemistry, 253(2), 162-168 (1997)
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