コンテンツへスキップ
Merck
  • Dosage Compensation of X-Linked Muller Element F Genes but Not X-Linked Transgenes in the Australian Sheep Blowfly.

Dosage Compensation of X-Linked Muller Element F Genes but Not X-Linked Transgenes in the Australian Sheep Blowfly.

PloS one (2015-10-28)
Rebecca J Linger, Esther J Belikoff, Maxwell J Scott
要旨

In most animals that have X and Y sex chromosomes, chromosome-wide mechanisms are used to balance X-linked gene expression in males and females. In the fly Drosophila melanogaster, the dosage compensation mechanism also generally extends to X-linked transgenes. Over 70 transgenic lines of the Australian sheep blowfly Lucilia cuprina have been made as part of an effort to develop male-only strains for a genetic control program of this major pest of sheep. All lines carry a constitutively expressed fluorescent protein marker gene. In all 12 X-linked lines, female larvae show brighter fluorescence than male larvae, suggesting the marker gene is not dosage compensated. This has been confirmed by quantitative RT-PCR for selected lines. To determine if endogenous X-linked genes are dosage compensated, we isolated 8 genes that are orthologs of genes that are on the fourth chromosome in D. melanogaster. Recent evidence suggests that the D. melanogaster fourth chromosome, or Muller element F, is the ancestral X chromosome in Diptera that has reverted to an autosome in Drosophila species. We show by quantitative PCR of male and female DNA that 6 of the 8 linkage group F genes reside on the X chromosome in L. cuprina. The other two Muller element F genes were found to be autosomal in L. cuprina, whereas two Muller element B genes were found on the same region of the X chromosome as the L. cuprina orthologs of the D. melanogaster Ephrin and gawky genes. We find that the L. cuprina X chromosome genes are equally expressed in males and females (i.e., fully dosage compensated). Thus, unlike in Drosophila, it appears that the Lucilia dosage compensation system is specific for genes endogenous to the X chromosome and cannot be co-opted by recently arrived transgenes.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
エチルアルコール(純粋), for molecular biology
Sigma-Aldrich
クロロホルム, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
ドデシル硫酸ナトリウム, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
Sigma-Aldrich
クロロホルム, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
ドデシル硫酸ナトリウム, ≥99.0% (GC), dust-free pellets
Sigma-Aldrich
ドデシル硫酸ナトリウム 溶液, BioUltra, for molecular biology, 10% in H2O
Sigma-Aldrich
エタノール, JIS special grade, ≥99.5%
Sigma-Aldrich
フェノール 溶液, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Sigma-Aldrich
クロロホルム, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
Sigma-Aldrich
10 mMトリス、pH 8.0、1 mM EDTAで飽和した25:24:1のフェノール:クロロホルム:イソアミルアルコール, Supplied with Equilibration buffer, for molecular biology
Sigma-Aldrich
ドデシル硫酸ナトリウム 溶液, BioUltra, for molecular biology, 20% in H2O
Sigma-Aldrich
クロロホルム, contains amylenes as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
クロロホルム, ReagentPlus®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
ドデシル硫酸ナトリウム, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
クロロホルム, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
エタノール, SAJ first grade, ≥99.5%
Sigma-Aldrich
フェノール 溶液, BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, for molecular biology
Sigma-Aldrich
Isoamyl alcohol, ≥98%, FG
Sigma-Aldrich
3-メチル-1-ブタノール, reagent grade, 98%
Sigma-Aldrich
リボヌクレアーゼA ウシ膵臓由来, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Supelco
ドデシル硫酸ナトリウム, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
クロロホルム, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
3-メチル-1-ブタノール, ACS reagent, ≥98.5%
Sigma-Aldrich
エチルアルコール(純粋), 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
ドデシル硫酸ナトリウム, ≥98.0% (GC)
Sigma-Aldrich
クロロホルム, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)
Sigma-Aldrich
ドデシル硫酸ナトリウム, ACS reagent, ≥99.0%
Sigma-Aldrich
エタノール, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
クロロホルム, JIS special grade, ≥99.0%
Sigma-Aldrich
フェノール, natural, 97%, FG