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  • Characterization and immunomodulatory activities of polysaccharides from Spirogyra neglecta (Hassall) Kützing.

Characterization and immunomodulatory activities of polysaccharides from Spirogyra neglecta (Hassall) Kützing.

Bioscience, biotechnology, and biochemistry (2015-05-15)
Utoomporn Surayot, JianGuo Wang, Ju Hun Lee, Chartchai Kanongnuch, Yuwadee Peerapornpisal, SangGuan You
要旨

Sulfated polysaccharides (SP) isolated from freshwater green algae, Spirogyra neglecta (Hassall) Kützing, and fractionated SPs were examined to investigate their molecular characteristics and immunomodulatory activity. The crude and fractionated SPs (F1, F2, and F3) consisted mostly of carbohydrates (68.5-85.3%), uronic acids (3.2-4.9%), and sulfates (2.2-12.2%) with various amounts of proteins (2.6-17.1%). D-galactose (23.5-27.3%), D-glucose (11.5-24.8%), L-fucose (19.0-26.7%), and L-rhamnose (16.4-18.3%) were the major monosaccharide units of these SPs with different levels of L-arabinose (3.0-9.4%), D-xylose (4.6-9.8%), and D-mannose (0.4-2.3%). The SPs contained two sub-fractions with molecular weights (Mw) ranging from 164 × 10(3) to 1460 × 10(3) g/mol. The crude and fractionated SPs strongly stimulated murine macrophages, producing considerable amounts of nitric oxide and various cytokines via up-regulation of their mRNA expression by activation of nuclear factor-kappa B and mitogen-activated protein kinases pathways. The main backbone of the most immunoenhancing SP was (1→3)-L-Fucopyranoside, (1→4,6)-D-Glucopyranoside, and (1→4)-D-Galactopyranoside.

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製品内容

Sigma-Aldrich
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D-(+)-グルコース, ≥99.5% (GC)
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D-(+)-グルコース, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
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硫酸, 99.999%
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デキストロース, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
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D-(+)-グルコース, ≥99.5% (GC), BioXtra
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重水素化ホウ素ナトリウム, 98 atom % D, 90% (CP)
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グリセロール 溶液, 83.5-89.5% (T)
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グリセロール, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
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グリセロール, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
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硫酸, SAJ first grade, ≥95.0%
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D-(+)-グルコース, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
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硫酸, JIS special grade, ≥95.0%
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グリセロール, FCC, FG
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硫酸 溶液, 1 M
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硫酸 溶液, 0.5 M
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D-(+)-グルコース, Hybri-Max, powder, BioReagent, suitable for hybridoma
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硫酸 溶液, 0.05 M
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D-(+)-グルコース, ACS reagent
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無水酢酸, ACS reagent, ≥98.0%
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グリセロール, BioXtra, ≥99% (GC)
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硫酸 溶液, 0.1 M
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グリセロール, SAJ first grade, ≥98.0%
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グリセロール, JIS special grade, ≥99.0%
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無水酢酸