コンテンツへスキップ
Merck
  • Folate-conjugated beta-cyclodextrin-based polymeric micelles with enhanced doxorubicin antitumor efficacy.

Folate-conjugated beta-cyclodextrin-based polymeric micelles with enhanced doxorubicin antitumor efficacy.

Colloids and surfaces. B, Biointerfaces (2014-07-25)
Lu Zhang, Jiafei Lu, Yangmin Jin, Liyan Qiu
要旨

In order to enhance the antitumor effects of doxorubicin (DOX), a novel micellar vector with high DOX loading and tumor targeting function based on folate-conjugated amphiphilic copolymer folate-poly(ethylene glycol)-poly(d,l-lactide)-β-cyclodextrin (FA-PEL-CD) was constructed. Cytotoxicity and cellular uptake experiments were performed in HeLa, KB, and A549 cell lines expressing different amounts of folate receptors in order to evaluate the targeting effect of the folate modification. The antitumor experiments performed in a KB cell-xenografted nude mouse model showed that the treatment with 10mg/kg DOX loaded FA-PEL-CD micelles achieved approximately 86% of tumor growth inhibition compared to the control. Ex vivo fluorescence imaging experiments and histological examination confirmed that folate modification can enhance the antitumorigenesis efficacy and reduce the cardiotoxicity of DOX. These results suggest that FA-PEL-CD copolymer-based micelles are promising nanocarriers for targeted doxorubicin delivery, with improved antitumor efficacy and reduced toxicity in normal tissues.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
ジメチルスルホキシド, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
ジメチルスルホキシド, ACS reagent, ≥99.9%
Sigma-Aldrich
トリフルオロ酢酸, ReagentPlus®, 99%
Sigma-Aldrich
トリフルオロ酢酸, suitable for HPLC, ≥99.0%
Sigma-Aldrich
ジメチルスルホキシド, for molecular biology
Sigma-Aldrich
ジクロロメタン, suitable for HPLC, ≥99.8%, contains amylene as stabilizer
Sigma-Aldrich
ジメチルスルホキシド, suitable for HPLC, ≥99.7%
Sigma-Aldrich
ジクロロメタン, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%
Sigma-Aldrich
ジメチルスルホキシド, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
トルエン, ACS reagent, ≥99.5%
Sigma-Aldrich
ジメチルスルホキシド, ReagentPlus®, ≥99.5%
Sigma-Aldrich
ジクロロメタン, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer
Sigma-Aldrich
トルエン, suitable for HPLC, 99.9%
Sigma-Aldrich
トルエン, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
トリフルオロ酢酸, puriss. p.a., suitable for HPLC, ≥99.0% (GC)
Sigma-Aldrich
ジメチルスルホキシド, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
ジメチルスルホキシド, puriss. p.a., ACS reagent, ≥99.9% (GC)
Sigma-Aldrich
4-(ジメチルアミノ)ピリジン, ReagentPlus®, ≥99%
Sigma-Aldrich
トルエン, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.7% (GC)
Sigma-Aldrich
ジクロロメタン, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)
Sigma-Aldrich
ジクロロメタン, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
トリフルオロ酢酸, ≥99%, for protein sequencing
Sigma-Aldrich
トリエチルアミン, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
ジメチルスルホキシド, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
ジクロロメタン, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)
Sigma-Aldrich
トルエン, ACS reagent, ≥99.5%
Sigma-Aldrich
ジメチルスルホキシド, PCR Reagent
Sigma-Aldrich
ジクロロメタン, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
ジクロロメタン, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
トルエン, Laboratory Reagent, ≥99.3%