コンテンツへスキップ
Merck
  • Interactions of connexin 43 and aquaporin-4 in the formation of glioma-induced brain edema.

Interactions of connexin 43 and aquaporin-4 in the formation of glioma-induced brain edema.

Molecular medicine reports (2014-11-07)
Gang Li, Xiaozhi Liu, Zhenlin Liu, Zhiguo Su
要旨

Connexin 43 (Cx43) and aquaporin-4 (AQP4) have important roles in the formation of glioma-induced brain edema; however, the association between these two factors in the development of edema has remained to be elucidated. In the present study, immunofluorescence and western blot analysis revealed that in a rat model of intracranial C6 glioma, Cx43 expression levels were low to undetectable and AQP4 expression levels were low in glioma cells. Significantly higher Cx43 and AQP4 levels were detected in the tissue surrounding the glioma. To further investigate the potential interaction between Cx43 and AQP4, normal glial cells and C6 glioma cells were cultured in hypotonic medium. Reverse transcription quantitative polymerase chain reaction indicated that AQP4 and Cx43 mRNA expression levels increased as a function of time in normal glial cells and C6 glioma cells in a hypotonic environment. However, the increase observed in normal glial cells was significantly lower than that observed in C6 glioma cells. Furthermore, AQP4 expression levels changed prior to alterations in Cx43 expression. Following AQP4 silencing in C6 cells, the increase in Cx43 expression was significantly attenuated (P<0.05). In normal cells, Cx43 silencing did not influence AQP4 expression (P>0.05). Therefore, it was hypothesized that AQP4 and Cx43 had two distinct mechanisms underlying brain edema formation within and surrounding the glioma. Cx43 may be a downstream effector of AQP4. The elucidation of this pathway may aid in the development of drugs targeting the interaction between AQP4 and Cx43, providing novel therapeutic possibilities for glioma-induced brain edema.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
酢酸, glacial, ACS reagent, ≥99.7%
Sigma-Aldrich
酢酸, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
酢酸, glacial, ≥99.99% trace metals basis
Sigma-Aldrich
酢酸, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Sigma-Aldrich
酢酸, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
Sigma-Aldrich
酢酸 溶液, suitable for HPLC
Sigma-Aldrich
酢酸, for luminescence, BioUltra, ≥99.5% (GC)
USP
氷酢酸, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
酢酸, ≥99.5%, FCC, FG
Sigma-Aldrich
5α-アンドロスタン-17β-オール-3-オン, ≥97.5%
Sigma-Aldrich
酢酸, natural, ≥99.5%, FG
Sigma-Aldrich
酢酸, ≥99.7%
Sigma-Aldrich
エチレンジアミン, purified by redistillation, ≥99.5%
Sigma-Aldrich
酢酸, JIS special grade, ≥99.7%
Sigma-Aldrich
エチレンジアミン, ReagentPlus®, ≥99%
Sigma-Aldrich
酢酸, glacial, puriss., 99-100%
Sigma-Aldrich
キシラジン, ≥99%
Sigma-Aldrich
5α-アンドロスタン-17β-オール-3-オン, purum, ≥99.0% (TLC)
Sigma-Aldrich
酢酸, SAJ first grade, ≥99.0%
Sigma-Aldrich
酢酸 溶液, 1 M, 1 N
Sigma-Aldrich
エチレンジアミン, BioXtra
Supelco
酢酸, analytical standard
Sigma-Aldrich
酢酸, ≥99.7%, SAJ super special grade
Sigma-Aldrich
エチレンジアミン 溶液, technical, 75-80%
Sigma-Aldrich
エチレンジアミン, puriss. p.a., absolute, ≥99.5% (GC)
Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
Supelco
エチレンジアミン, analytical standard
Sigma-Aldrich
酢酸, ≥99.7%, for titration in non-aqueous medium
Sigma-Aldrich
酢酸, 99.5-100.0%
Sigma-Aldrich
エチレンジアミン, meets USP testing specifications