electron microscopy: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells
Monoclonal Anti-U2AF65 (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF65. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF65) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF65kDa subunit is a non-snRNP protein component of spliceosome complexes.
免疫原
組み換えヒトU2AF65。
アプリケーション
Monoclonal Anti-U2AF65 antibody produced in mouse has been used in:
RNA immunoprecipitation
immunoblotting
chromatin immunoprecipitation (ChIP)
enzyme-linked immunosorbent assay (ELISA)
immunohistochemistry
immunocytochemistry
immunoelectron microscopy
生物化学的／生理学的作用
U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.
物理的形状
0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site specification is controlled by interactions of cis-acting determinants on a transcript with specific RNA binding proteins. These interactions are frequently localized to the intronic U-rich polypyrimidine tracts
The role of splicing factor mutations in the pathogenesis of the myelodysplastic syndromes
Boultwood J, et al.
Advances in Biological Regulation, 54(32), 153-161 (2014)
Splicing of human pre-mRNA is reciprocally coupled to 3' end formation by terminal exon definition, which occurs co-transcriptionally. It is required for the final maturation of most human pre-mRNAs and is therefore important to understand. We have used several strategies
Spliceosome structure and function
Will CL and Luhrmann R
Cold Spring Harbor Perspectives in Biology, 3(7), a003707-a003707 (2011)
Gene expression profiling of U2AF2 dependent RNA-protein interactions during CD4+ T cell activation
