SRP6001

Procathepsin K human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• 別名: CTSK, CTSO, CTSO2
• CAS番号: 177529-06-5
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥95% (SDS-PAGE)
• フォーム: liquid
• 包装: pkg of 10 μg
• 濃度: ~200 μg/mL
• 不純物: Endotoxin, tested
• NCBIアクセッション番号: P43235
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... CATK(1513)

詳細

詳細

Procathepsin K is the inactive proenzyme of cathepsin K.[1] Cathepsin K is a lysosomal cysteine protease and part of the papain cysteine protease family.[2] It is expressed in osteoclasts.[3][4] The gene encoding this protein is localized on human chromosome 1q21.[5]

生物化学的／生理学的作用

Cathepsin K has a role in bone resorption and remodeling.[1][4] It has a triple helical collagen hydrolase activity.[6] Mutations in the gene encoding this protein have been linked to pycnodysostosis.[7]

物理的形状

Liquid (25 mM Tris pH 8.0, 500 mM NaCl)

その他情報

Procathepsin K can be activated by adjusting the pH 4.0 by adding an equal volume of 100 mM NaAc pH 3.9, 10 mM DTT, 5 mM EDTA followed by incubation for 40 min at RT. Activated mature Cathepsin is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, we recommend to add methyl methanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) and to freeze the sample in liquid nitrogen or on dry ice. The hydrophobic thiol-reactive compound MMTS modifies cysteine′s by attaching its relatively small, uncharged thiomethyl-blocking group to reactive sulfhydryl groups (Nishimura et al., 1975). This reversible reaction arrests the auto-proteolytic process. The activity of the enzyme can be restored to nearly unmodified levels by adding L-cysteine (3M excess over MMTS) to the enzyme solution.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable