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342001

Procathepsin K, Human, Recombinant, E. coli

Name: Procathepsin K, Human, Recombinant, <i>E. coli</i>
Brand: MM

Recombinant, human procathepsin K expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site.

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この商品について

EC Number:

3.4.22.38 (BRENDA, IUBMB)

NACRES:

NA.77

UNSPSC Code:

12352202

Form:

liquid

Assay:

≥95% (SDS-PAGE)

Recombinant:

expressed in E. coli

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特性

recombinant

expressed in E. coli

Quality Segment

100

assay

≥95% (SDS-PAGE)

form

liquid

specific activity

≥1000 mU/mg protein

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

詳細

General description

If the activated enzyme is not used immediately, it is recommended to add methyl methanthiosulfonate (1 mM final concentration MMTS).

Note: 1 mU = 1 milliunit.

Recombinant, human procathepsin K (amino acids 19-329) (GenBank target symbol = S79895, ACC No. P43235) expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Cathepsin K, a member of the papain superfamily of cysteine proteinases, plays an important role in osteoclast-mediated bone resorption and collagen degradation. Cathepsin K is synthesized as an inactive proenzyme that is converted to its mature, active form by proteolytic cleavage of the 99 amino acid propeptide domain. Inhibitors of cathepsin K include leupeptin (Cat. No. 108975) (IC₅₀ = 70 nM), E-64 (Cat. No. 324890) (IC₅₀ = 5 nM), and cystatin (Cat. No. 324891 or 324896). Requires activation prior to use.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 500 mM NaCl, 25 mM Tris, pH 8.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C). Following activation the enzyme is unstable and should include MMTS for storage (see recommended reaction conditions for activation).

Other Notes

McQueney, M., et al. 1997. J. Bio. Chem.272, 13955.
Bossard, M., et al. 1996. J. Biol. Chem.271, 12517.
Drake, F., et al. 1996. J. Biol. Chem.271, 12511.
Bromme, D. and Okamoto, K. 1995. Biol. Chem. Hoppe-Seyler376, 379.
Baron, R. 1989 Anat. Rec.224, 317.
Littlewood-Evans, A.J., et al. 1975. Cancer Res.57, 5386.
Nishimura, J.S. et al. 1975. Arch. Biochem. Biophys.170, 461.

One unit is defined as the amount of enzyme that will hydrolyze 1 µmole benzyloxycarbonyl-phenylalanine-arginine-7-amido-4-methylcoumarin per min at 37°C, pH 5.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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当該品目	219461	539735	575001
Sigma-Aldrich 342001 Procathepsin K, Human, Recombinant, E. coli	Sigma-Aldrich 219461 Cathepsin K, His•Tag^®, Human, Recombinant, E. coli	Sigma-Aldrich 539735 PTP1B, Human, Recombinant, E. coli	Sigma-Aldrich 575001 α-Synuclein, Human, Recombinant, E. coli
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay ≥95% (SDS-PAGE)
form liquid	form liquid	form liquid	form liquid
storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles
specific activity ≥1000 mU/mg protein	specific activity ≥900 mU/mg protein	specific activity ≥20 U/mg	specific activity -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c