SRP0252

ULK Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥40% (SDS-PAGE)

• 別名: ATG1
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in FreeStyle^™ 293-F cells
• アッセイ: ≥40% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 113 kDa
• 包装: pkg of 10 μg
• メーカー／製品名: Invitrogen
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_003565
• UniProtアクセッション番号: O75385
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... ULK1(8408)

詳細

詳細

ULK1 (unc-51 like autophagy activating kinase 1) is a Ser/Thr protein kinase which forms a component of the ULK1 protein kinase complex. This protein complex also contains the adaptor proteins ATG (autophagy-related) 13, RB1CC1/FIP200 (RB1-inducible coiled-coil 1), and ATG101. ULK1 protein is one of the five orthologs of yeast Atg1 found in mammals.[1]

生物化学的／生理学的作用

ULK1 (unc-51 like autophagy activating kinase 1) protein kinase plays a crucial role in the control of autophagy initiation. This protein is responsible for activating a cascade of signaling pathways which ultimately results in the formation of phagosomes. The phosphorylation of ULK1 by MTORC1 (mammalian target of rapamycin complex) leads to the negative regulation of autophagy.[1][2] Activation of type I interferon (IFN) receptor (IFNR) results in the activation of ULK1 protein, which, in turn, results in the transcription of gene in a IFN-stimulated response elements (ISRE) and IFNγ activation site (GAS) elements-mediated manner. It is also required for the control of the expression of key IFN-stimulated genes (ISGs).[3]

物理的形状

Formulated in 2 5mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。

法的情報

FreeStyle is a trademark of Invitrogen Corp.