Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
￥20,300
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使用法
1 sufficient for 10 preparations
テクニック
DNA extraction: suitable
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詳細
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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アプリケーション
特徴および利点
- Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
- Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
- Capable of extracting miRNA with high purity with no detectable large RNA.
- Hazardous organic extractions are not involved.
法的情報
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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労働安全衛生法名称等を通知すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
Xiaoqiong Tang et al.
Cancer management and research, 12, 2119-2125 (2020-04-14)
LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the
Ying Zhang et al.
Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
MicroRNAs control mRNA fate by compartmentalization based on 3? UTR length in male germ cells
Zhang Y, et al.
Genome Biology, 18(1), 105-105 (2017)
Identification of microRNA-mRNA regulatory network associated with oxidative DNA damage in human astrocytes
Nwokwu CD, et al.
ASN Neuro, 14, 17590914221101704-17590914221101704 (2022)
関連コンテンツ
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
Sigma-Aldrich®アドバンストゲノミクスは、CRISPR、Cas9、合成ガイドRNA（sgRNA）、およびジンクフィンガーヌクレアーゼ（ZFN）などの遺伝子編集とサイレンシング工学技術の有力なプロバイダーです。
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
1 回答-
役に立ちましたか？
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
1 回答-
Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
役に立ちましたか？
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
1 回答-
We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
役に立ちましたか？
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
1 回答-
We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
役に立ちましたか？
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