SAB4200810

Anti-Podocin, Mouse monoclonal

clone PD-65, purified from hybridoma cell culture

• 別名: NPHS2
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: PD-65, monoclonal
• フォーム: buffered aqueous solution
• 分子量: ~42 kDa
• 交差性: bovine, rat, human, mouse
• 包装: antibody small pack of 25 μL
• 濃度: 1 mg/mL
• テクニック: immunoblotting: 0.25-0.5 μg/mL using using human HEK-293T cells.; immunofluorescence: 1-2 μg/mL using human HEK-293T cells.
• アイソタイプ: IgG2a
• UniProtアクセッション番号: Q9NP85
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NPHS2(7827)

詳細

詳細

Podocytes are highly specialized visceral epithelial cells that cover the outer aspect of the glomerulus in the kidney. The foot processes of adjacent podocytes regularly interdigitate leaving filtration slits that are bridged by a thin slit diaphragm. This is an extracellular adherens-like junction that serves as a barrier to macromolecules and plays a crucial role in the glomerular filtration process. Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band-7-stomatin family of lipid raft-associated proteins. It is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It interacts there with nephrin (NPHS1 protein), a protein critical in development and function of the kidney filtration barrier and the adapter protein CD2-AP. Podocin is anchored in the glycosphingolipids and cholesterol rich lipid rafts of the outer leaflet of the plasma membrane and is partially localized with the actin cytoskeleton. In vitro studies suggest its involvement in nephrin signaling facilitation via AP-1 in HEK cells. In addition to its presence in the kidney, it was also reported in rat nervous tissue and possibly in vascular smooth muscle.

免疫原

Synthetic peptide of human Podocin , conjugated to KLH

アプリケーション

The antibody may be used in various immunochemical techniques including Immunobloting (~42kDa) and Immunofluorescence. Detection of the Podocin band by Immunoblotting is specifically inhibited by the immunogen.a

物理的形状

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative

その他情報

This product is for R&D use only, not for drug, household, or other uses.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable