R1158

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

• 別名: RNAse inhibitor
• MDL番号: MFCD00132187
• UNSPSCコード: 12352200
• NACRES: NA.52

特性

• リコンビナント: expressed in E. coli
• 品質水準: 300
• グレード: for molecular biology
• フォーム: buffered aqueous solution
• 分子量: ~50 kDa
• 濃度: 20-40 units/μL
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

アプリケーション

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

• cDNA synthesis from mRNA
• reverse transcriptase polymerase chain reaction (RT-PCR)
• in vitro transcription and translation
• mRNA protection from degradation[1]
• formaldehyde cross-linked lncRNA purification[2]
• RNA-fluorescence in situ hybridization (FISH)[3]

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

生物化学的／生理学的作用

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

構成

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

単位の定義

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

物理的形状

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

その他情報

• Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
• This product is for R&D use only, not for drug, household, or other uses.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable