R1008

モノクロナール抗ウサギIgG (γ-鎖特異的) マウス宿主抗体

clone RG-96, ascites fluid

• 別名: モノクロナール抗ウサギIgG (γ-鎖特異的)
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: secondary antibodies
• クローン: RG-96, monoclonal
• 含みます: 15 mM sodium azide
• 交差性: rabbit
• 以下との反応禁止: goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
• テクニック: dot blot: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: 1:2,000; western blot: suitable
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

本製品はウサギIgGのγ（重）鎖に位置するエピトープを認識します。イムノブロットでは、本抗体は未変性および変性したウサギIgGを認識します。ELISAでは、本抗体はウサギIgGに特異的に反応しますが、ウサギIgAおよびIgMまたはヒトIgG、IgAおよびIgMとは交差反応しません。以下の生物種のIgGとも交差反応しません：ウシ、ネコ、ニワトリ、イヌ、ヤギ、モルモット、ウマ、ブタ、ラットまたはヒツジ。

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

特異性

Monoclonal Anti-Rabbit IgG is specific for an epitope located on the heavy (γ) chain of rabbit IgG, in an immunoblot of denatured, reduced rabbit IgG. The antibody does not react with rabbit IgA, IgM or light chains. No cross reactivity is observed with IgG from bovine, cat, chicken, dog, goat, guinea pig, horse, human, pig, rat or sheep.

アプリケーション

Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse has been used in

• enzyme linked immunosorbent assay (ELISA)
• dot blots
• immunohistology
• western blotting
• peptide mass fingerprinting (PMF)

生物化学的／生理学的作用

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc region is involved in the development of therapeutic antibody.

物理的形状

The product is provided as ascites fluid with 15 mM sodium azide (see MSDS) as a preservative.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable