P3818

プロテインジスルフィドイソメラーゼ ウシ肝臓由来

≥100 units/mg protein, lyophilized powder

• 別名: PDI
• CAS番号: 37318-49-3
• Enzyme Commission number: 5.3.4.1 (BRENDA, IUBMB)
• MDL番号: MFCD00163364
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• フォーム: lyophilized powder
• 品質水準: 200
• 比活性: ≥100 units/mg protein
• 分子量: 107 kDa
• 組成: Protein, ~10% Lowry
• 保管温度: −20°C
• SMILES記法: [S](=O)(=O)(ON)c1c(cc(c(c1)C(=O)O)Cl)Cl
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
• InChI Key: DHUYKLYJBKXDBM-UHFFFAOYSA-N

詳細

詳細

Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2.[1] The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose.[2] PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.[3]

アプリケーション

Protein Disulfide Isomerase from bovine liver has been used:

• to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi[3]
• in the in vitro translation reaction for the generation of disulfide bonds[4]
• in insulin-disulfide reduction assay and peptide binding assay[5]
• as a positive control in thiol-disulfide oxidoreductase activity assay[6]

生物化学的／生理学的作用

Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges.[4] It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.[7]

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.[8]

包装

容量は、タンパク質含量に基づいています。

物性

ウシ肝臓由来のタンパク質ジスルフィドイソメラーゼ(PDI)は、分子量107 kDa(ゲル濾過)のホモ二量体ですが、単量体の分子量は57 kDa(SDS-PAGE)であると報告されています。この酵素は、総炭水化物含量12%の糖タンパク質であり、マンノース4.6%、ガラクトース2.5%、NANA1.4%、2-アセトアミド-2-デオキシグルコース3.5%で構成されています。

単位の定義

1ユニットは、ジチオスレイトール存在下、pH 7.5、25°C、1分間で、1.0 mg/mLのインスリン溶液のA₆₅₀に0.01の変化を生じさせる酵素量です。

物理的形状

凍結乾燥品(リン酸カリウムバッファ-塩および安定剤含有)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)