Based on previous results, a 20% difference in methylation is typically observable. It is recommended to conduct replicate experiments, assess the margin of error (technical and/or biological), and utilize a p-test or other statistical approach to evaluate its applicability in your specific case.
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詳細
DNA全体におけるメチル化部位の移動を短時間で確実に測定するためのキットです。メチル化DNAの相対的レベルの検出に必要な試薬はすべて付属しています。まず、最大で200 ngの精製DNAがストリップ上のウェルに結合します。捕捉用・検出用の両抗体によりメチル化DNAを検出し、次に比色法により定量します。吸光度の測定値がメチル化DNAの量に比例することを利用してサンプル中のメチル化DNAの量を求めます。
特徴および利点
- ELISAベースの形式。
- 4つの簡単な手順で完了します。
- コントロール用のメチル化DNAを含め、すべての試薬が付属します。
- 検出限界は、完全メチル化DNA約5 ng。
- 使用されるDNAは、10〜200 ng程度。
- 手順は3.5時間で完了します。
- 96ウェル形式により、単一サンプルの研究またはハイスループット研究が可能。
- 5-mCに対して高い特異性を持つ最適化された抗体および試薬。非メチル化シトシンとの交差反応性はありません。
- 接着細胞や浮遊細胞、組織、血漿、唾液、尿、血清などの他の体液でも使用できます。
法的情報
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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危険有害性情報
危険有害性の分類
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
保管分類コード
8A - Combustible corrosive hazardous materials
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
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資料
The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay.
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
プロトコル
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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Could you please provide the sensitivity of the kit and explain what differences in methylation status it can detect?
1 回答-
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When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, can I dilute the entire antibody at once and save it for later?
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No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
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Can I use chloroform to extract my DNA before using Product MDQ1, Imprint® Methylated DNA Quantification Kit?
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Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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How does the storage temperature relate to shipping conditions?
1 回答-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Is Product MDQ1, Imprint® Methylated DNA Quantification Kit, shipped in two parts?
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Yes, Product MDQ1, Imprint® Methylated DNA Quantification Kit, is shipped in 2 parts. Part 1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
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When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, do I need to use all the wells in the plate at once?
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No, you can use as many wells as needed. You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
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Are there any species restrictions for Product MDQ1, Imprint® Methylated DNA Quantification Kit?
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The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
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What is the detection limit of Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 回答-
The detection limit of this kit is 5 nanograms of fully methylated DNA.
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