The principle of this kit is the reaction of formaldehyde with acetoacetanilide in the presence of ammonia. Neither a MESNA (4-Morpholineethanesulfonic acid, sodium) buffer nor EDTA in the test sample would be expected to interfere with this reaction. Having said that, it would be appropriate to run a blank containing MESNA and EDTA, and standards spiked with MESNA and EDTA, to confirm they they are not interfering with the test result...that is, making it either artificially high or low.
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使用法
sufficient for 100 fluorometric tests
検出方法
fluorometric
関連疾患
cancer
保管温度
2-8°C
詳細
Formaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent.[1] Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. Increased production of formaldehyde has been observed in many cancer cell lines and increased formaldehyde concentration in urine has been associated with bladder and prostrate cancer.[2]
アプリケーション
- Modified Natural Rubber as a Simple Chemical Sensor with Smartphone Detection for Formaldehyde Content in a Seafood Sample.: This article explores the use of modified natural rubber as a chemical sensor, paired with smartphone detection, to measure formaldehyde content in seafood. This approach highlights the potential for accessible and mobile detection systems in food safety applications (Yeerum et al., 2022).
- Optical enzymatic formaldehyde biosensor based on alcohol oxidase and pH-sensitive methacrylic-acrylic optode membrane.: The study develops an optical enzymatic biosensor for formaldehyde, using alcohol oxidase with a pH-sensitive membrane, offering a novel approach for quick and sensitive detection in various analytical applications (Nurlely et al., 2022).
特徴および利点
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
適合性
Suitable for the detection of formaldehyde in urine and other biological samples.
原理
The Formaldehyde Assay kit provides a simple and direct procedure for measuring formaldehyde levels in urine and other biological samples. In this assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia resulting in a fluorescent (λex = 370/λem = 470 nm) product, proportional to the formaldehyde present.
シグナルワード
Danger
危険有害性の分類
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 1 - STOT SE 3
ターゲットの組織
Eyes,Central nervous system, Respiratory system
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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労働安全衛生法名称等を通知すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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試験成績書（COA）
Lot/Batch Number
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Cristina A Nadalutti et al.
Scientific reports, 10(1), 5575-5575 (2020-03-30)
Formaldehyde (FA) is a simple biological aldehyde that is produced inside cells by several processes such as demethylation of DNA and proteins, amino acid metabolism, lipid peroxidation and one carbon metabolism (1-C). Although accumulation of excess FA in cells is
Formaldehyde in the workplace and in the home: exploring its clinical toxicology.
Montanaro A.
Laboratory Medicine, 27(11), 752-758 (2015)
Lysine-specific demethylase 1 in breast cancer cells contributes to the production of endogenous formaldehyde in the metastatic bone cancer pain model of rats.
Liu J, et al.
PLoS ONE, 8(3), e58957-e58957 (2013)
Wancang Liu et al.
Frontiers in microbiology, 11, 1988-1988 (2020-09-26)
Traditional diosgenin manufacturing process has led to serious environmental contamination and wastewater. Clean processes are needed that can alternate the diosgenin production. The β-glucosidase FBG1, cloned from Fusarium sp. CPCC 400709, can biotransform trillin and produce diosgenin. In this study
Joan Artigas et al.
Environmental pollution (Barking, Essex : 1987), 259, 113951-113951 (2020-01-16)
Environmental dissolved organic matter (DOM) has been proved to increase microbial population sizes and stimulate the degradation of some pesticide molecules. Among these molecules, the present study investigated the biodegradation of the herbicide glyphosate depending on photoautotrophs DOM supply in
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Is the assay compatible with MES buffer? Is EDTA affecting the reaction?
1 回答-
役に立ちましたか？
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What is the limit of detection that the MAK131 Formaldehyde Assay is capable of?
1 回答-
The kit is capable of detecting formaldehyde down to 1.5 micromolar or 45 ppb. The upper end of detection for undiluted samples is 100 micromolar, which is the highest concentration of the standard curve.
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アクティブなフィルタ
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