microarray: suitable western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine
H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.
The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.
アプリケーション
Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.
生物化学的／生理学的作用
H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..
物理的形状
0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。
免責事項
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As like in mammalian system, the DNA damage responsive cell cycle checkpoint functions play crucial role for maintenance of genome stability in plants through repairing of damages in DNA and induction of programmed cell death or endoreduplication by extensive regulation
Resveratrol decreases Rad51 expression and sensitizes cisplatin-resistant MCF-7 breast cancer cells
In all organisms, DNA damage must be repaired quickly and properly, as it can be lethal for cells. Because eukaryotic DNA is packaged into nucleosomes, the structural units of chromatin, chromatin modification is necessary during DNA damage repair and is
