16-202A

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

clone JBW301, Upstate^®, from mouse

• 別名: H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: FITC conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: JBW301, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Upstate^®
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_002105.2
• UniProtアクセッション番号: P16104
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: phosphorylation (pSer139)
• 遺伝子情報: human ... H2AX(3014)

詳細

詳細

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

特異性

Broad species cross-reactivity is expected based on conservation of sequence homology.

Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.

免疫原

Epitope: Ser139

KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン

Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton^™ X-100 in TBS (5 min.).

Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.

品質

Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

ターゲットの説明

15 kDa

物理的形状

Protein G Purified

Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.

保管および安定性

Stable for 1 year at from date of receipt.

アナリシスノート

Control
Staurosporine-treated Jurkat cells

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable