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由来生物
mouse
品質水準
結合体
biotin conjugate
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
JBW301, monoclonal
化学種の反応性
human
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
phosphorylation (pSer139)
遺伝子情報
human ... H2AX(3014)
詳細
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
特異性
A broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, Mr 15kDa.
免疫原
Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate is a Mouse Monoclonal Antibody for detection of phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone & has been validated in ICC & WB.
品質
Routinely evaluated in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine (19-123).
Western Blot Analysis:
0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
Western Blot Analysis:
0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
ターゲットの説明
15 kDa
物理的形状
Protein G Purified
Purified biotin conjugated mouse monoclonal IgG1 in buffer containing 70% storage buffer (0.02 M Phosphate buffer, pH7.6, 0.25 M NaCl, 0.1% sodium azide) and 30% glycerol
保管および安定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
アナリシスノート
Control
Staurosporine-treated Jurkat cell lysates.
Staurosporine-treated Jurkat cell lysates.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
16-193:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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