H5527

ハイグロマイシン B 溶液 Streptomyces hygroscopicus由来

≥60% (HPLC), 45-60 mg/mL in H₂O, γ-irradiated

• 別名: Hygromycin b, Hygrovetine
• 実験式（ヒル表記法）: C₂₀H₃₇N₃O₁₃
• CAS番号: 31282-04-9
• 分子量: 527.52
• Beilstein: 6755837
• MDL番号: MFCD06795479
• UNSPSCコード: 51102829
• PubChem Substance ID: 24895698
• NACRES: NA.85

特性

• 由来生物: Streptomyces hygroscopicus
• 品質水準: 200
• 無菌性: γ-irradiated
• 濃度: ≥60% (HPLC); 45-60 mg/mL in H₂O
• 色: yellow-brown to orange
• 抗生物質活性スペクトル: viruses
• 作用機序: protein synthesis | interferes
• 保管温度: 2-8°C
• SMILES記法: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI Key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

詳細

詳細

Chemical structure: aminoglycoside

アプリケーション

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

生物化学的／生理学的作用

作用機序：この製品は、翻訳を阻害する能力によって原核生物でのm-RNA鋳型の誤読を誘導し、タンパク質合成を阻害することによって作用します。

抗菌スペクトル：ハイグロマイシンBは細菌、真菌および高等真核生物細胞に作用します。

注意

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

調製ノート

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H₂O, and γ-irradiated .

安全性情報

• ピクトグラム: GHS06,GHS08,GHS05
• シグナルワード: Danger
• 危険有害性情報: H301 + H311,H318,H330,H334
• 注意書き: P260 - P280 - P284 - P301 + P310 - P305 + P351 + P338 - P310
• 危険有害性の分類: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Resp. Sens. 1
• 保管分類コード: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter