SBR00039

Hygromycin B

Min. 95% (HPLC ELSD) Ready Made Solution, 50 mg/mL in water (from Streptomyces hygroscopicus)

• 別名: Hygromycin B solution from Streptomyces hygroscopicus
• 実験式（ヒル表記法）: C₂₀H₃₇N₃O₁₃
• CAS番号: 31282-04-9
• 分子量: 527.52
• Beilstein: 6755837
• MDL番号: MFCD06795479
• UNSPSCコード: 51102829
• NACRES: NA.76

特性

• product name: ハイグロマイシン B 溶液 Streptomyces hygroscopicus由来, 50 mg/mL in water (from Streptomyces hygroscopicus)
• 由来生物: Streptomyces hygroscopicus
• 品質水準: 200
• アッセイ: ≥95%
• 形状: liquid
• 濃度: 50 mg/mL in water (from Streptomyces hygroscopicus)
• 抗生物質活性スペクトル: Gram-negative bacteria; Gram-positive bacteria; fungi; viruses
• 作用機序: protein synthesis | interferes
• 保管温度: 2-8°C
• SMILES記法: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI Key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

詳細

詳細

Hygromycin B, also known as Hydrovetine and D-streptamine, is a distinctive aminoglycoside antibiotic recognized for its ability to impede the growth of both prokaryotic microorganisms, such as bacteria, and eukaryotic microorganisms like yeasts and mammalian cells. Its mechanism of action involves reinforcing the interaction of peptidyl-tRNA in the ribosomal acceptor ("A") site, ultimately hindering the translocation process mediated by EF-2 (elongation Factor-2). Derived from Streptomyces hygroscopicus, this aminoglycoside antibiotic serves as a valuable selection agent for cells harboring the hygromycin-resistant gene, known as hph or hyg.

Widely utilized for selection and maintenance purposes, Hygromycin B showcases its inhibitory prowess on protein synthesis, contributing to its role in the selection and upkeep of both prokaryotic and eukaryotic cells featuring the hygromycin resistance gene. This antibiotic demonstrates anthelmintic activity in addition to its impact on translational processes in ribosomes across diverse organisms. Its specificity in inhibiting translocation, as well as its influence on decoding fidelity, makes Hygromycin B a valuable tool for the selection of transfected cells containing the Escherichia coli hygromycin resistance gene (hyg or hph) and for maintaining the Hygromycin phenotype in resistant cells. The monophasic inhibition of polypeptide synthesis suggests a single-site binding of Hygromycin B to the ribosome, adding a layer of specificity to its mode of action, making it indispensable for various facets of scientific research in cell biology and biochemical research.

アプリケーション

Hygromycin B. has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreadin. as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/m. specifically at 100 μg/mL for prokaryote. 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes

生物化学的／生理学的作用

作用機序：この製品は、翻訳を阻害する能力によって原核生物でのm-RNA鋳型の誤読を誘導し、タンパク質合成を阻害することによって作用します。

抗菌スペクトル：ハイグロマイシンBは細菌、真菌および高等真核生物細胞に作用します。

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

特徴および利点

• High-quality antibiotic suitable for multiple research applications
• Ideal for Cell Biology, Metabolomics, and Biochemical research
• Ready-made solution for ease of use
  

その他情報

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安全性情報

• ピクトグラム: GHS06
• シグナルワード: Danger
• 危険有害性情報: H301,H312,H330
• 注意書き: P260 - P264 - P270 - P280 - P302 + P352 + P312 - P304 + P340 + P310
• 危険有害性の分類: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal
• 保管分類コード: 6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3