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包装
pack of 1 column
メーカー/製品名
Cytiva 28-9924-74
パラメーター
1.2 ml/min - 2.3 ml/min flow rate
44 psi
ベッドサイズ
7.7 mm × 100 mm
ベッド容積
4.7 mL
キャパシティ
~25 mg binding capacity(human serum albumin/ml medium)
保管温度
2-8°C
関連するカテゴリー
詳細
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
アプリケーション
HiScreen™ Capto™ Blue is prepacked with the base matrix Capto™ to which Cibacron Blue 3G is covalently bound via a hydrophilic spacer immobilized with a stable amine bond. Capto™ Blue is highly chemically stable and has a more rigid Agarose base matrix than Blue Sepharose™ 6 Fast Flow. This allows the use of faster flow rates and larger sample volumes, leading to higher throughput and improved process economy. The application area is the same as for Blue Sepharose™ Fast FLow (e.g., purification of albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons, and related Proteins). The main benefit of Capto™ Blue compared to Blue Sepharose™ 6 Fast FLow is its improved chemical stability provided with a new coupling chemistry involving a spacer arm. It tolerates repeated cleaning-in-place and sanitization at both high and Low pH as well as autoclaving. Also the leakage of Cibacron Blue is reduced as compared to Blue Sepharose™ 6 Fast Flow.
特徴および利点
- Prepacked with Capto™ Blue
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
その他情報
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法的情報
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
プロトコル
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)