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Merck
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安全性情報

38733

Sigma-Aldrich

レムリ細胞溶解バッファー

non smelling

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About This Item

UNSPSCコード:
12161700
NACRES:
NA.25
現在、価格および在庫状況を閲覧できません。

フォーム

liquid

組成

bromophenol blue, 0.004%
DTT, 400 mM
glycerol, 20%
SDS, 4%
TRIS, 0.125 M

pH

~6.8

適合性

in accordance for electrophoresis test

保管温度

−20°C

関連するカテゴリー

アプリケーション

レムリ溶解バッファーは、SDSゲル電気泳動のタンパク質サンプルの調整に用いられる無臭溶解バッファーです。

ピクトグラム

CorrosionExclamation mark
GHS05,GHS07

シグナルワード

Danger

危険有害性情報

H302,H315,H318

危険有害性の分類

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

PRTR

第一種指定化学物質

Jan Code

38733-1ML:
38733-5X2ML:
38733-2ML:
38733-5ML:
38733-VAR:
38733-BULK:

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
BCCF6315
BCBS0040V
BCBQ4821V
BCBM0474V
BCBJ1244V

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特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

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Slide 1 of 3

1 of 3

トリス-グリシン-SDSバッファー, 10倍濃縮液

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T7777

トリス-グリシン-SDSバッファー, 10倍濃縮液

ゲルローディングバッファー for NA electrophoresis

Sigma-Aldrich

G2526

ゲルローディングバッファー

Sigma-Aldrich

Sigma-Aldrich

S7705

TRAPeze™1X CHAPS溶解バッファー

Sean R Gallagher
Current protocols in protein science, Chapter 10, Unit 10-Unit 10 (2012-04-04)
One-dimensional gel electrophoresis of proteins provides information about the molecular size, amount, and purity of a protein sample. Separated proteins can be recovered from polyacrylamide gels for subsequent characterization by a variety of secondary techniques, such as mass spectrometry to
Xiaoqiang Wu et al.
Analytical biochemistry, 421(1), 347-349 (2011-11-01)
A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can
Vincent Archambault et al.
Genes & development, 22(19), 2707-2720 (2008-10-04)
The conserved Polo kinase controls multiple events in mitosis and cytokinesis. Although Polo-like kinases are regulated by phosphorylation and proteolysis, control of subcellular localization plays a major role in coordinating their mitotic functions. This is achieved largely by the Polo-Box
Sean R Gallagher
Current protocols in immunology, Chapter 8, Unit 8-Unit 8 (2008-04-25)
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can also serve to purify proteins for use in further
Lei Liu et al.
Experimental and therapeutic medicine, 13(2), 461-466 (2017-03-30)
Cancer-induced bone pain can severely compromise the life quality of patients, while tolerance limits the use of opioids in the treatment of cancer pain. Monocyte chemoattractant protein-1 (MCP-1) is known to contribute to neuropathic pain. However, the role of spinal
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質問

1–7/7 質問  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか？

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか？

  3. What is the difference between the two Laemmli buffers S3401 and 38733?

    1 回答

    1. Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.  So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).

      役に立ちましたか？

  4. What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?

    1 回答

    1. The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).  Some people are very sensitive to the odor of 2-mercaptoethanol.

      役に立ちましたか？

  5. Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?

    1 回答

    1. An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.

      役に立ちましたか？

  6. Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?

    1 回答

    1. For most applications, it is considered to be a 2X solution.

      役に立ちましたか？

  7. What is the Department of Transportation shipping information for this product?

    1 回答

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか？

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