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ligand
protein G
包装
pack of 2 × 1 mL
メーカー/製品名
Cytiva 28-9670-66
保管条件
(20% Ethanol, 10% medium slurry)
パラメーター
Room temperature temp. range
Matrix
paramagnetic, spherical, highly cross-linked agarose particles
キャパシティ
27 mg binding capacity(human IgG/ml gel)
保管温度
2-8°C
関連するカテゴリー
詳細
アプリケーション
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.
特徴および利点
- Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
- High purity and yield
- Robust parallel screening of antibodies with high reproducibility
- Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
- Visible and dense Sepharose™ based magnetic beads give increased simplicity for handling
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
プロトコル
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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