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ligand
p-aminobenzamidine
包装
pack of 25 mL
メーカー/製品名
Cytiva 17-5123-10
保管条件
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
Matrix
highly cross-linked 4% agarose
平均直径
90 μm
cleaning in place
1-9
動作範囲
2-8
保管温度
2-8°C
関連するカテゴリー
詳細
アプリケーション
特徴および利点
- Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
- p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
- For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
プロトコル
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to prepare samples for purification with affinity chromatography.
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