コンテンツへスキップ
Merck
すべての画像(2)

主要文書

GE17-0405-01

HiTrap® Protein G High Performance

Cytiva 17-0405-01, pack of 1 × 5 mL

別名:

Protein G affinity column, Protein purification column

ログイン組織・契約価格を表示する
すべての画像(2)

About This Item

UNSPSCコード:
23151816
NACRES:
NA.56
現在、価格および在庫状況を閲覧できません。

ligand

recombinant protein G lacking albumin-binding region

包装

pack of 1 × 5 mL

メーカー／製品名

Cytiva 17-0405-01

保管条件

(20% Ehtanol)
 (20% Ethanol)

パラメーター

flow rate
42 psi

ベッドサイズ

16 mm × 25 mm

ベッド容積

5 mL

カラム内径

16 mm

Matrix

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-9

動作範囲

3-9

キャパシティ

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 125 mg binding capacity(human IgG/column)

保管温度

2-8°C

関連するカテゴリー

詳細

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame
GHS02

シグナルワード

Warning

危険有害性情報

H226

保管分類コード

3 - Flammable liquids

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 資料 section.

サポートが必要な場合は、お問い合わせください カスタマーサポート

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Wioleta M Zelek et al.
Immunology, 161(2), 103-113 (2020-06-20)
The implication of complement in multiple diseases over the last 20 years has fuelled interest in developing anti-complement drugs. To date, the focus has been on C5; blocking cleavage of C5 prevents formation of two pro-inflammatory activities, C5a anaphylatoxin and membrane

資料

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

すべて表示

プロトコル

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）