We have no information on storing this product in the freezer. Yes, it is best to spin your cell culture sample down before the assay. Yes, you will need to add the reagent to your standards.
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詳細
Griess試薬はGriess反応に基づいています。構成成分には、水に懸濁したナフチルエチレンジアミン二塩酸塩およびリン酸に溶解したスルファニルアミドが含まれます。この試薬を試料中の亜硝酸塩と反応させると紫色のアゾ色素が生成し、その吸光度を波長546 nmで測定します。[1]
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Dam. 1 - Skin Corr. 1
保管分類コード
8A - Combustible corrosive hazardous materials
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
G4410-10G:
G4410-VAR:
G4410-BULK:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids
Green LC, et al.
Analytical biochemistry, 126(1), 131-138 (1982)
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Kasetty G, et al.
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1. Can the griess reagent solution be stored for longer if I keep it in the freezer? 2. Does my sample (a supernatant of cell culture media) has to be centrifuged first before I do the assay? 3. Do I add the reagent to my standard (NaNO2) as well?
1 回答-
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if we dissolve Griess reagent 40 mg/ml in water then what will be its concentration (molarity).
1 回答-
This product is a multi-component reagent, thus a molarity is not available. When prepared according to instructions, the prepared solution will contain 0.288% (w/v) of sulfanilamide and 0.016% (w/v) naphthylethylene diaminedihydrochloride. In addition, the product also includes an unreported amount of phosphoric acid.
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How many grams of reagent powder should be dissolved in 1 ml of distilled water?
1 回答-
This product is tested for solubility at 40 mg/mL in water. The product is intended to be reconstituted in the bottle by adding 250 ml of water. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdf役に立ちましたか？
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Since this is a solid, how much quantity of water it should be diluted with?
1 回答-
The 10 g quantity is packaged in a 250 mL bottle. To reconstitute, simply add 250 mL of distilled water.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdf役に立ちましたか？
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how the limit of detection ?
1 回答-
According to T.P. Misko, et al., Analytical Biochemistry, 214, 11-16 (1993), this Griess Reagent, in fluorometric testing, detects to at least 10 nM nitrite. This information has not been validated.
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What is advised for disposal if a solution is made up with H2O?
1 回答-
Please refer to the SDS for disposal information. Per section 13.1, Waste material must be disposed of in accordance with the national and loc No mixing with other waste. Handle uncleaned containers like the product. See www.retrologistik.com for processes regarding the return of chemicals and containers, or contact technical service if you have further questions. https://www.sigmaaldrich.com/sds/sigma/g4410.
Please contact your EHS team or or disposal service group, If there's none, we recommend a national disposal company such as Cleanharbors at https://www.cleanharbors.com/.
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When using Product G4410, Griess' reagent (modified), what product should I use to make a nitrite standard curve?
1 回答-
Sodium nitrite (Product No. S2252 or 237213) can be used to make a standard curve. A typical standard curve would cover the range of 0-100 microMolar nitrite.
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Is there an enzymatic method to convert nitrate to nitrite?
1 回答-
A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996). For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Will phenol red interfere with the Griess reaction Product G4410, Griess' reagent (modified)?
1 回答-
While phenol red does not interfere with the Griess reaction, it will interfere with the absorption reading taken at 540nm. It is recommended to use media without phenol red to avoid any interference in the absorption readings.
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