DUO92103

Duolink^® In Situ Red Starter Kit Mouse/Goat

• 別名: in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit
• UNSPSCコード: 41116158
• NACRES: NA.32

特性

• 製品種目: Duolink^®
• 品質水準: 100
• テクニック: proximity ligation assay: suitable
• 蛍光検出: λ_ex 594 nm; λ_em 624 nm (red) (Texas Red^®; Zeiss Filter set 31)
• 適合性: suitable for fluorescence
• 保管温度: −20°C

詳細

アプリケーション

Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink^® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), red detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink^® In Situ Red Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Red fluorescence detection reagents are often used with Texas Red^® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

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Duolink^® In Situ Red Starter Kit Mouse/Goat has been used in the critical commercial assay[1] and proximity ligation assay (PLA).[2]

特徴および利点

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

法的情報

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Texas Red is a registered trademark of Life Technologies

キットの構成要素は別途購入可能です。

• DUO92006 Duolink^® In Situ PLA^® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
• DUO92001 Duolink^® In Situ PLA^® Probe Anti-Mouse PLUS
• DUO92008 Duolink^® In Situ Detection Reagents Red
• DUO82049 Duolink^® In Situ Wash Buffers, Fluorescence
• DUO82040 Duolink^® In Situ Mounting Medium with DAPI

安全性情報

• ピクトグラム: GHS08,GHS05,GHS09
• シグナルワード: Danger
• 危険有害性情報: H290,H317,H334,H411
• 注意書き: P234 - P261 - P272 - P273 - P280 - P302 + P352
• 危険有害性の分類: Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
• 保管分類コード: 8A - Combustible corrosive hazardous materials
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable