D9281

λDNA , EcoR I Hind III 消化

for DNA electrophoresis

• UNSPSCコード: 41141827
• NACRES: NA.25

特性

• フォーム: liquid
• 使用法: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• 適合性: suitable for electrophoresis (DNA)
• 保管温度: −20°C

詳細

詳細

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

アプリケーション

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.[1]

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

構成

10mM Tris-HCl溶液 (pH 8.0, 1mM EDTA含有)

その他情報

125–21,226 bpの13フラグメントを含有

For optimal resolution, the recommended agarose gel concentration is 0.7%.

調製ノート

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable