V79

86041102, hamster lung, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: V79, 86041102, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• 由来生物: hamster lung
• 包装: tube of 5 μg 86041102-DNA-5UG; pkg of vial of cells 86041102-1VL
• 成長モード: Adherent
• 核型: 2n=22
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Hamster Chinese lung male

細胞株の説明

Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. A clone called V79.4 is available (Sigma Catalogue number. 93010723). V79 is well established in toxicology studies. Stability of karyotype and morphology is making them suitable for gene toxicity assays with low background aberrations. The cells do not express endogenous cytochrome P450 and have been used for the insertion and expression of P450 from human, rat, mouse and fish.

アプリケーション

Growth factors and transformation studies, mutagenesis study

V79 has been used to detect structural and numerical chromosome damage by measuring the formation of micronuclei in interphase cells that have been through a mitotic division.[1]

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:12 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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