おすすめの製品
由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
DCS-310, monoclonal
フォーム
buffered aqueous solution
分子量
antigen 54 kDa
化学種の反応性
human
濃度
~2 mg/mL
テクニック
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
アイソタイプ
IgG2b
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CHEK1(1111)
関連するカテゴリー
詳細
Checkpoint kinase 1 (Chk1) protein is a serine/threonine-protein kinase with a molar mass of 54 kDa. Monoclonal Anti-Chk1 (mouse IgG2b isotype) is derived from the DCS-310 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human Chk1.
免疫原
リコンビナントヒトChk1。
アプリケーション
Monoclonal Anti-Chk1 antibody produced in mouse has been used in:
- immunoprecipitation
- immunohistochemistry
- immunoblotting
Monoclonal Anti-Chk1 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoprecipitation, microarray and western blot at a working concentration of 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells. It was used for immunohistochemistry in a study to investigate the specific role of Chk1 phosphorylations in cell survival and checkpoint activation. It was used for immunohistochemistry at a working dilution of 1:4000 in a study to explore Chk1 knockdown as a novel therapeutic approach to arrest cell-cycle progression in MM cells, thus increasing the rate of cell death. It was used for the immunoprecipitation and detection of total Chk1 by western blot to study a possible regulatory relationship between Chk1 and CK1, a member of the casein kinase 1 family.
生物化学的／生理学的作用
Checkpoint kinase 1 (Chk1) protein acts as a cell cycle checkpoint regulator.
The protein propagates signals from damaged or unreplicated DNA. It is expressed and active only in S-G2 phases of the cell cycle. In response to DNA damage, it phosphorylates CDC25C at Ser 216, thus preventing activation of the Cdc2-cyclin B complex and mitotic entry, blocking cell cycle progression. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Chk1 can phosphorylate Wee1, a negative regulator of G2 to M transition.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C9358-BULK:
C9358-.2ML:
C9358-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
C Lukas et al.
Cancer research, 61(13), 4990-4993 (2001-06-30)
The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase
Hironori Abe et al.
Human molecular genetics, 27(7), 1136-1149 (2018-01-24)
The continuity of life depends on mechanisms in the germline that ensure the integrity of the genome. The DNA damage response/checkpoint kinases ATM and ATR are essential signaling factors in the germline. However, it remains unknown how a downstream transducer
Identification of potential therapeutic targets in malignant mesothelioma using cell-cycle gene expression analysis
Romagnoli S, et al.
The American Journal of Pathology, 174(3), 762-770 (2009)
Solange Romagnoli et al.
The American journal of pathology, 174(3), 762-770 (2009-02-17)
Cell-cycle defects are responsible for cancer onset and growth. We studied the expression profile of 60 genes involved in cell cycle in a series of malignant mesotheliomas (MMs), normal pleural tissues, and MM cell cultures using a quantitative polymerase chain
A Blasina et al.
Current biology : CB, 9(1), 1-10 (1999-01-16)
In human cells, the mitosis-inducing kinase Cdc2 is inhibited by phosphorylation on Thr14 and Tyr15. Disruption of these phosphorylation sites abrogates checkpoint-mediated regulation of Cdc2 and renders cells highly sensitive to agents that damage DNA. Phosphorylation of these sites is
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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