C3860

シトクロムP450 ヒト

1B1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

• Enzyme Commission number: 1.6.2.4 (BRENDA, IUBMB)
• MDL番号: MFCD03456520
• UNSPSCコード: 12161501
• NACRES: NA.47

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• フォーム: solution
• 分子量: 45-60 kDa
• 包装: vial of 0.5 nmol
• 適合性: suitable for molecular biology
• UniProtアクセッション番号: Q16678
• アプリケーション: cell analysis
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... CYP1B1(1545)

詳細

詳細

Research Area: Immuno and CKS
Cytochromes are a type of protein belonging to superfamilies containing heme as a cofactor, making them hemoproteins. These proteins, also known as CYPs, serve as substrates in enzymatic reactions. The term "P450" originates from the enzyme′s maximum optical density peak at 450 nm when in its reduced state combined with carbon monoxide. Cytochromes are present in various tissues throughout the human body, with a predominant presence in intestinal and hepatic tissues.[1]

アプリケーション

Cytochrome P450 human has been used in aflatoxin B1 (AFB1) metabolites analysis to study its effects on AFB1-induced cytotoxicity and micronuclei formation in human hepatocyte (L-02) and human lymphoblastoid (TK6) cells.[2] It has also been used in cell-free cytochrome P4501B1 (CYP1B1) inhibition assays.[3]

生物化学的／生理学的作用

シトクロムP450は、異種アイソザイムのファミリーであり、その主な機能は、中間代謝(脂肪酸など)の一機能として、および外因性化合物(薬物または毒物)を解毒するために、低分子を酸化することです。一部のアイソフォームは、対象の狭い基質特異性を有しますが、他のアイソフォームには特異性はみられません。

Cytochromes play a crucial role in metabolizing a wide range of xenobiotic compounds and are engaged in diverse functions such as steroid metabolism, deactivation of drugs and procarcinogens, metabolism of fatty acids, detoxification of xenobiotic substances, and breakdown of exogenous compounds. It facilitates the oxidation of substances using iron. CYP is well-established that changes in CYP enzymes often occur as a common consequence of immune system activation following infection and inflammation. Despite the overlapping effects of cytokines, the activity of human P450s is independently regulated in infection and inflammation.[1]

単位の定義

One unit will reduce 1 nanomole of cytochrome C per minute at pH 7.4 at 37 deg C.

物理的形状

100mM リン酸カリウムバッファ-溶液 (pH 7.4)。

調製ノート

ミクロソ-ムにはヒトCYP1B1およびリコンビナントヒトNADPH-P450レダクタ-ゼが含まれています。

アナリシスノート

エトキシレゾルフィンの7-脱エチル化に対する触媒能を試験しています。

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable