C4982

シトクロムP450 ヒト

3A4 isozyme microsomes, with P450 reductase and cytochrome b₅, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

• 別名: Cytochrome P450 enzyme, Human cytochrome P450, P450 enzyme
• UNSPSC Code: 12161501
• NACRES: NA.47
• EC Number: 1.6.2.4 (BRENDA, IUBMB)
• MDL number: MFCD03456520
• Biological source: human
• Recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• Concentration: ≥2 mg/mL (Bichinchonic acid method)

特性

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• form: solution
• mol wt: 45-60 kDa
• packaging: vial of ~0.5 nmol, vial of ≥25 units
• concentration: ≥2 mg/mL (Bichinchonic acid method)
• technique(s): co-immunoprecipitation (co-IP): suitable
• solubility: water: soluble
• suitability: suitable for molecular biology
• UniProt accession no.: P08684
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CYP3A4(1576)

詳細

General description

Research area: IMMUNO AND CKS

Cytochrome P450 (CYP450) is a membrane-bound hemoprotein that constitutes a vast superfamily of heme-thiolate proteins engaged in the metabolism of exogenous and endogenous compounds. These CYP450 enzymes feature an active heme iron center that is bound to a protein molecule via a highly conserved cysteine thiolate ligand.

Application

Cytochrome P450 human has been used in in vitro binding assays to understand protein dimerization via haem–haem stacking and its significance in cancer.[1] It has also been used to study in vitro binding between PGRMC1 and CYP3A4.[2]

Biochem/physiol Actions

シトクロムP450は、異種アイソザイムのファミリーであり、その主な機能は、中間代謝(脂肪酸など)の一機能として、および外因性化合物(薬物または毒物)を解毒するために、低分子を酸化することです。一部のアイソフォームは、対象の狭い基質特異性を有しますが、他のアイソフォームには特異性はみられません。

Cytochrome P450 (CYP) enzymes are associated with many reactions including O-dealkylation, epoxidation, S-oxidation, and hydroxylation.

Physical form

100 mMリン酸カリウムバッファー溶液(pH 7.4)。

Other Notes

1ユニットは、pH 7.4、37°Cで1分あたり1 nmolのテストステロンを6β-ヒドロキシテストステロンに変換する酵素量です。

ミクロソ-ムには、ヒト組換え体CYP3A4、組み換えウサギNADPH-P450還元酵素が含まれます。

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable