CelLytic B cannot be used with the MBP system. We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.
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アプリケーション
バクテリア細胞を溶解し、組み換えタンパク質の抽出に用いる独自組成の両性イオン界面活性剤で、タンパク質を変性させません。
特徴および利点
- 超音波破砕やリゾチ-ム処理等の従来法より高効率でタンパク質抽出ができます。
- ト 1~25 gのバクテリア細胞ペ-ストに使用できます。
- アフィニティ-クロマトグラフィ-、IP、ウェスタンブロット等その後のアプリケ-ションにに影響を与えません。
- プロテア-ゼインヒビタ-、インヒビタ-カクテル、カオトロ-プ、塩、キレ-ト剤、還元剤と一緒に使用できます。
その他情報
40 mM Trizma® HCl（pH 8.0）で調製した界面活性剤配合物の2倍濃縮液です。
法的情報
米国特許番号7,282,475 B2によって保護されており、研究目的の使用に限り販売しています。商用目的の場合は、別途ライセンスを取得する必要があります。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
B7310-250ML:
B7310-VAR:
B7310PROC:
B7310-50ML:
B7310-BULK:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Russel M Vincent et al.
ACS synthetic biology, 8(4), 675-685 (2019-03-12)
Using engineered initiator tRNA for precise control of protein translation within cells has great promise within future orthogonal translation systems to decouple housekeeping protein metabolism from that of engineered genetic systems. Previously, E. coli strain C321.ΔA. exp lacking all UAG stop
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Can I use CelLytic B with the maltose binding protein system (MBP System)?
1 回答-
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Can I concentrate my sample that was made using CelLytic B?
1 回答-
If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications. heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.
役に立ちましたか？
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Can I obtain protein from inclusion bodies with CelLytic B?
1 回答-
If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies. CelLytic B is used to isolate the inclusion bodies from the bacteria. CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.
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Can I use CelLytic B for eukaryotic cells?
1 回答-
CelLytic B was optimized for bacterial cells. It may lyse some mammalian cells. CelLytic B 10X is a better choice for the lysis of yeast and mammalian cells due to the increased detergent concentration.
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Can I use the CelLytic™ B cell lysate in an activity assay?
1 回答-
CelLytic B contains a zwitterionic detergent so the protein is active and can be used in protein activity assays.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus (Product Nos. CB0050 and CB0500)?
1 回答-
CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.
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When using the CelLytic™ B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?
1 回答-
When using the CelLytic™ B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent. A 1:20 dilution would be even better, as long as the protein level remains within the standard curve.
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What is the difference between CelLytic B (Product No. B7435), CelLytic B 2x (Product No. B7310) and CelLytic B 10x (Product No. C8740)?
1 回答-
CelLytic B 2X has double the strength of CelLytic B for small volume extractions. This allows CelLytic B 2X to be used when a higher protein concentration or lower volume is required. The original formula, CelLytic B, requires 10-20 ml of the reagent for 1 gram of wet cell paste. Only 5 ml of the CelLytic B 2X reagent is required to lyse and extract protein from 1 gram of wet cell paste. CelLytic B 10X is provided as a concentrated mixture of the proprietary detergents found in CelLytic B, without any buffering component. This allows the user to customize the extraction reagent by choosing the detergent concentration and buffering components ideal for the extraction and purification of the protein(s) of interest. CelLytic B 10X is also suitable for the lysis of yeast and mammalian cells.
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What bacterial cells can be used with CelLytic B?
1 回答-
CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21. It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.
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