C8740 does not interfere with downstream applications such as 6X HisTag affinity tag purification, or detection on a Western Blot with an anti-6X His antibody.
If the intention is to conjugate a protein to a 6X HisTag, the protein should first be purified from the lysate before conjugation. C8740 will not interfere with a conjugation protocol after the protein of interest has been isolated from the lysate.
C8740
セルリティック™ B細胞溶解試薬
For bacterial cell lysis, 10× concentrate
別名:
bacterial cell lysis reagent
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
10 mL | 在庫from市川市大町368 | ￥21,300 |
50 mL | 在庫from市川市大町368 | ￥63,800 |
100 mL | 在庫from市川市大町368 | ￥110,000 |
この商品について
￥21,300
在庫詳細
Quality Segment
form
liquid
Application
バクテリア細胞を溶解し、組み換えタンパク質の抽出に用いる独自組成の両性イオン界面活性剤で、タンパク質を変性させません。
TM[="CelLytic"] B細胞溶解試薬は、大腸菌5-ヘリックスバンドルタンパク質の溶解に使用されています。また、肝炎B X-結合タンパク質（HBXIP）および大腸菌TG1細胞の溶解にも適します。
Features and Benefits
- 超音波破砕やリゾチ-ム処理等の従来法より高効率でタンパク質抽出ができます。
- ト 1~25 gのバクテリア細胞ペ-ストに使用できます。
- アフィニティ-クロマトグラフィ-、IP、ウェスタンブロット等その後のアプリケ-ションにに影響を与えません。
- プロテア-ゼインヒビタ-、インヒビタ-カクテル、カオトロ-プ、塩、キレ-ト剤、還元剤と一緒に使用できます。
Other Notes
CelLytic Bの10倍濃度の界面活性剤で、緩衝液を含有していないので実験系に用いる溶解試薬を柔軟に選択することが可能です。組み換えタンパク質の抽出と精製の自動プロトコ-ルにも適用可能です。
Legal Information
米国特許番号7,282,475 B2によって保護されており、研究目的の使用に限り販売しています。商用目的の場合は、別途ライセンスを取得する必要があります。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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当該品目 | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form solution | form solution |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
保管分類
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Can this product safe to be used for downstream crosslinking stains such as a 6x HIsTag?
1 回答-
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What bacterial cells can be used with CelLytic B?
1 回答-
CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21. It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.
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When using the CelLytic™ B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?
1 回答-
When using the CelLytic™ B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent. A 1:20 dilution would be even better, as long as the protein level remains within the standard curve.
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Can I obtain protein from inclusion bodies with CelLytic B?
1 回答-
If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies. CelLytic B is used to isolate the inclusion bodies from the bacteria. CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.
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Can I use CelLytic B with the maltose binding protein system (MBP System)?
1 回答-
CelLytic B cannot be used with the MBP system. We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.
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Can I use CelLytic B for eukaryotic cells?
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CelLytic B was optimized for bacterial cells. It may lyse some mammalian cells. CelLytic B 10X is a better choice for the lysis of yeast and mammalian cells due to the increased detergent concentration.
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What is the difference between CelLytic B (Product No. B7435), CelLytic B 2x (Product No. B7310) and CelLytic B 10x (Product No. C8740)?
1 回答-
CelLytic B 2X has double the strength of CelLytic B for small volume extractions. This allows CelLytic B 2X to be used when a higher protein concentration or lower volume is required. The original formula, CelLytic B, requires 10-20 ml of the reagent for 1 gram of wet cell paste. Only 5 ml of the CelLytic B 2X reagent is required to lyse and extract protein from 1 gram of wet cell paste. CelLytic B 10X is provided as a concentrated mixture of the proprietary detergents found in CelLytic B, without any buffering component. This allows the user to customize the extraction reagent by choosing the detergent concentration and buffering components ideal for the extraction and purification of the protein(s) of interest. CelLytic B 10X is also suitable for the lysis of yeast and mammalian cells.
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Can I concentrate my sample that was made using CelLytic B?
1 回答-
If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications. heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.
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What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus (Product Nos. CB0050 and CB0500)?
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CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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