B2059

ビオチンポリエチレンオキシドヨードアセトアミド

• 別名: ビオチンPEOヨードアセトアミド
• 実験式（ヒル表記法）: C₁₈H₃₁IN₄O₅S
• 分子量: 542.43
• MDL番号: MFCD04974830
• UNSPSCコード: 12352119
• PubChem Substance ID: 24891627
• NACRES: NA.32

特性

• 詳細: Sulfhydryl specific, water soluble biotinylation reagent
• フォーム: powder
• 溶解性: H₂O: 10 mg/mL
• 保管温度: 2-8°C
• SMILES記法: ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12
• InChI: 1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)
• InChI Key: LWGLKVINXNMNJX-UHFFFAOYSA-N

詳細

詳細

Biotin polyethyleneoxide (PEO) iodoacetamide is a sulfhydryl (thiol) specific biotinylation reagent.[1]

アプリケーション

• Incorporates a 12 atom hydrophilic spacer.
• Typically coupled to sulfhydryl groups at pH 7.5 - 8.5
• Especially useful for cysteine labeling in many proteomics type applications such as peptide mapping and mass spectrometry.

Biotin Polyethyleneoxide Iodoacetamide has been used as a thiol reactive reagent to demonstrate the presence of free thiols in capsid protein L1 of human papillomavirus 16.[2] It has been used for biotinylation of proteins for ELISA (enzyme-linked immunosorbent assay) competition and pull down experiments.[3][4]

生物化学的／生理学的作用

Biotin polyethyleneoxide (PEO) iodoacetamide group reacts specifically with reduced thiols at pH 7.5 - 8.5. Peptides and small molecules containing thiol groups may also be labeled using this reagent. The biotin PEO iodoacetamide enables tagging of cysteine residues and conjugation to sulfhydryls in proteins making it useful in proteomics, peptide mapping, phosphopeptide analysis, and mass spectrometry.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H315,H319,H335
• 注意書き: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): dust mask type N95 (US), Eyeshields, Gloves