T. gondii-感染マウスの血清中の抗体の検出は、二次抗体としてペルオキシダーゼ結合抗マウス多価免疫グロブリン抗体を使用したELISAにより行われました。 特異的Mabに対する相対結合親和性は、PBSTで1:10,000に希釈し、21~23°Cで1時間インキュベートしたペルオキシダーゼ結合ヤギ抗マウス多価抗体を使用した阻害ELISAにより明らかにされました。
物理的形状
1%ウシ血清アルブミンと保存剤を含む0.01Mリン酸緩衝生理食塩水(pH 7.4)溶液
調製ノート
Wilson, M.B.およびNakane, P.K.によって報告された過ヨウ素酸法で作製しています(Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978))。
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Formation of anti-Toxoplasma gondii HSP70 (TgHSP70) antibody cross-reactive to mouse HSP70 (mHSP70) was observed in the sera of BALB/c (a resistant strain) and C57BL/6 (B6; a susceptible strain) mice after peroral infection with T. gondii cysts of the Fukaya strain.
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The humoral responses of Ebola virus (EBOV) survivors mainly target the surface glycoprotein GP, and anti-GP neutralizing antibodies have been associated with protection against EBOV infection. In order to elicit protective neutralizing antibodies through vaccination a native-like conformation of the