94742

バナジル リボヌクレオシド複合体 溶液

BioReagent, for molecular biology, 200 mM

• 別名: リボヌクレオシド バナジル複合体
• UNSPSCコード: 12352200
• NACRES: NA.52

特性

• グレード: Molecular Biology; for molecular biology
• 製品種目: BioReagent
• フォーム: liquid
• 濃度: 200 mM
• 不純物: oxovanadium V
• 適合性: in accordance for ribonuclease inhibition test
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]

Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase).[2] Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.[3]

アプリケーション

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.

Vanadyl ribonucleoside complexes solution has been used:

• as a component of polysome lysis buffer[4] and fluorescence in situ hybridization (FISH) buffer[5][6]
• as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes[7]

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable