This product is considered salt free. Following purification by Ion exchange chromatography, the material is precipitated in ethanol, washed, and finally dialyzed against water prior to lyophilization.
製品 R1753 は現在購入できません。 製品に関するお問い合わせはこちら（テクニカルサービス）
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アプリケーション
リボ核酸、トランスファー大腸菌（Type XX、W株）由来は、液体カラムクロマトグラフィー又は高速液体クロマトグラフィー（HPLC）などの方法による特定のアミノアシルtRNA種精製のための出発物質として使用されることがあります。
単位の定義
1 unitは 1.0 mLの水中(光路長は1 cm)でA260が1.0となる量です。
アナリシスノート
各製品は、アミノアシル-tRNAシンテターゼおよびアミノ酸混合物(Ala、Asp、Gly、Ile、Lys、Pro、Thr、Val、Arg、Glu、His、Leu、Phe、Ser、Tyr)を用いたアミノ酸アクセプター活性の評価済みです。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
R1753-2KU:
R1753-10KU:
R1753-100UN:
R1753-500UN:
R1753-VAR:
R1753-BULK:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Roya Pedram Fatemi et al.
Journal of biomolecular screening, 20(9), 1132-1141 (2015-07-16)
Long non-protein coding RNAs (lncRNAs) are an important class of molecules that help orchestrate key cellular events. Although their functional roles in cells are not well understood, thousands of lncRNAs and a number of possible mechanisms by which they act
Miguel López-Estepa et al.
PloS one, 10(4), e0118606-e0118606 (2015-04-22)
Cyclic N6-threonylcarbamoyladenosine ('cyclic t6A', ct(6)A) is a non-thiolated hypermodification found in transfer RNAs (tRNAs) in bacteria, protists, fungi and plants. In bacteria and yeast cells ct(6)A has been shown to enhance translation fidelity and efficiency of ANN codons by improving
Fuxiang Wei et al.
Nature communications, 11(1), 4902-4902 (2020-10-01)
Living cells and tissues experience various complex modes of forces that are important in physiology and disease. However, how different force modes impact gene expression is elusive. Here we apply local forces of different modes via a magnetic bead bound
Kirill Kondratov et al.
Journal of extracellular vesicles, 9(1), 1743139-1743139 (2020-04-29)
The aim of this study was to investigate cell source-dependent nucleic acids repertoire of diverse subpopulations of plasma extracellular vesicles (EVs). Blood plasma from nine healthy volunteers was used for the analysis. Samples of EVs were obtained by differential centrifugation
Thomas E Sladewski et al.
eLife, 7 (2018-06-27)
We investigated the role of full-length Drosophila Bicaudal D (BicD) binding partners in dynein-dynactin activation for mRNA transport on microtubules. Full-length BicD robustly activated dynein-dynactin motility only when both the mRNA binding protein Egalitarian (Egl) and K10 mRNA cargo were
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R1753 Ribonucleic acid,CAS Number 9014-25-9, transfer from Escherichia coli. Is it desalted?
1 回答-
役に立ちましたか？
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R1753 Ribonucleic acid,CAS Number 9014-25-9, transfer from Escherichia coli. Is it purified E. coli tRNA?
1 回答-
The product is isolated from Escherichia coli. It is soluble in water at 2 mg/ml, producing a clear, colorless solution.
役に立ちましたか？
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What is the meaning of "type XX" in R1753?
1 回答-
There are three classes of RNA: Messenger, Ribosomal, and Transfer. The type designations on products offered by Sigma are in-house designations and do not represent any specific biochemical significance. These designations can refer to the order of addition of the product to the catalog, source of material, method of preparation, differences in specifications, and so on.
役に立ちましたか？
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I am confused about this reagent based on the application vs. analysis note. Does this product contain deacylated tRNA or is it a mixture of various aminoacylated tRNAs?
1 回答-
This product is just deacylated tRNA. It is tested for tRNA for multiple amino acids.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Can Product R1753, tRNA, be used in molecular biology applications?
1 回答-
This product has not been tested for Dnase or RNase activity. Product Nos. R5636 and R8508 are from baker's yeast; they have been tested for DNase and Nickase.
役に立ちましたか？
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How many mgs of tRNA, Product R1753, come in the 100UN, 500UN, 2KU and 10KU package sizes?
1 回答-
The amount of powder will vary between lots depending on the activity. For example, a Certificate of Analysis of a lot may say 18 A260 units/mg solid. This means that the 100UN package will contain approxomately 5.56 mg of solid.
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アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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