Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.
特徴および利点
Ready-to-use buffer
No measuring and pH adjusting needed
Save time and minimize effort
Biological tests: free of DNase, RNase, protease, and nickase
Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
包装
Foil pouches
再構成
Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
保管および安定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
その他情報
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法的情報
pHast Pack is a trademark of Sigma-Aldrich Co. LLC