PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• 別名: 1X Bis-Tris transfer buffer, Bis-Tris
• UNSPSCコード: 41105319
• NACRES: NA.25

特性

• 製品種目: pHast Pack^™
• 品質水準: 100
• フォーム: powder
• 保管条件: (Tightly closed. Dry)
• pH: 7.2
• 微量陽イオン: Pb: ≤5 ppm
• その他の活性: DNAse, free; NICKase, free; Protease, free; RNAse, free
• 保管温度: room temp

詳細

詳細

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

アプリケーション

Bis-Tris buffer may be used in western blot as a transfer buffer.

特徴および利点

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

包装

Foil pouches

再構成

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

保管および安定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

その他情報

For additional information on our range of Biochemicals, please complete this form.

法的情報

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• ピクトグラム: GHS05
• シグナルワード: Danger
• 危険有害性情報: H318
• 注意書き: P280 - P305 + P351 + P338
• 危険有害性の分類: Eye Dam. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3