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使用法
sufficient for 5 96-well plate(s)
品質水準
包装
pkg of 1 kit
メーカー／製品名
Roche
保管条件
protect from light
蛍光検出
λex 361 nm; λem 486 nm (nuclei dye)
λex 490 nm; λem 515 nm (viable dye)
λex 535 nm; λem 617 nm (dead dye)
検出方法
fluorometric
輸送温度
dry ice
保管温度
−20°C (−15°C to −25°C)
関連するカテゴリー
詳細
The Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.
The kit is designed for 5 × 96 reactions for use in medium to high throughput analysis of cell behavior.
- Determine total cell number using fluorescence staining of cell nuclei.
- Fluorimetrically determine the percentage of dead cells based on membrane permeability.
- Verify the labeling of viable cells quantified by fluorescence staining using a vital dye
アプリケーション
Cell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.
特徴および利点
Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:
- No risk of losing cells in the one step assay: There are no fixation or washing steps.
- Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
- Obtain highly reproducible results using automated imaging and data evaluation with Roche′s Cellavista System.
包装
1 kit containing 3 components.
調製ノート
Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 °C. Contents can be frozen and thawed at least 5 times after preparation of solution.
保管および安定性
Store at -15–-25 °C. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)
その他情報
For life science research only. Not for use in diagnostic procedures.
キットの構成要素のみ
製品番号
詳細
- Nuclei Dye (blue cap) for fluorimetric determination of cell count based on labeling of cell nuclei
- Dead Dye (red cap) for fluorimetric determination of dead cells based on cell permeability
- Viable Dye ( green cap) for fluorimetric determination of viable cells based on metabolic activity
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Muta. 2
保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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