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詳細
Moesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. The MSN encoded protein moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, the MSN encoded protein is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. The MSN encoded Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane. EMD-Millipore’s Anti-MSN, clone 2C12 has been tested in western blots against purified recombinant protein as well as multiple human cancer cell lysates. The clone has also been tested successfully in paraffin embedded immunohistochemistry on human colon tissue and by flow cytometry on human Jurkat cells and ELISA using purified recombinant antigen.
免疫原
Purified protein fragment of human MSN expressed in E. coli.
アプリケーション
Detect Moesin using this mouse monoclonal antibody, Anti-MSN Antibody, clone 2C12 validated for use in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MSN in human tonsil and colon tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MSN in Jurkat cells.
Optimal working dilutions must be determined by end user.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MSN in Jurkat cells.
Optimal working dilutions must be determined by end user.
品質
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MSN in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MSN in 10 µg of Jurkat cell lysate.
ターゲットの説明
~68 kDa observed. Uncharacterized bands may appear in some lysate(s).
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シグナルワード
Danger
危険有害性情報
危険有害性の分類
Aquatic Chronic 3 - Repr. 1A
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABT456:
試験成績書（COA）
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アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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