ECM506
QCM Chemotaxis Cell Migration Assay, 24-well (5 µm), colorimetric
This 3 um QCM Chemotaxis Assay 24-well plate -colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.
別名:
Cell migration assay, Chemotaxis assay
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About This Item
UNSPSCコード:
12352207
eCl@ss:
32161000
NACRES:
NA.84
Pricing and Inventory is not available. Please check the Merck website for more information.
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品質水準
交差性（ホモロジーによる予測）
all
メーカー／製品名
Chemicon®
QCM
テクニック
activity assay: suitable
cell based assay: suitable
検出方法
colorimetric
輸送温度
wet ice
関連するカテゴリー
詳細
Also available: Cell Comb Scratch Assay! Get biochemical data from a scratch assay!Click Here
Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). Most cells are sized from 30 to 50 µm can migrate through 3 to 10 µm pore. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted. Most migration assays utilize an 8 µm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Millipore 5 µm QCM Chemotaxis Assay 24-well- colorimetric utilizes a 5 µm pore size, which is appropriate for a subset of fibroblast cells or cancer cells such as NIH-3T3 and MDA-MAB 231 cells. Cells migrated toward chemoattractants were measured by crystal violet, a nucleic dye, and quantified by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, and chemokinesis.
The Millipore QCM 5 µm Chemotaxis Assay 24-well-colorimetric provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
• QCM 8µm 24-well Chemotaxis Cell Migration Assays (ECM508, 509)
• QCM 5µm 24-well Chemotaxis Cell Migration Assay- Fluorometric (ECM507)
• QCM 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
• QCM 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
• QCM 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
• QCM 96-well ECMatrix Cell Invasion Assay (ECM555)
• QCM 96-well Collagen-based Cell Invasion Assay (ECM556)
Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). Most cells are sized from 30 to 50 µm can migrate through 3 to 10 µm pore. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted. Most migration assays utilize an 8 µm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Millipore 5 µm QCM Chemotaxis Assay 24-well- colorimetric utilizes a 5 µm pore size, which is appropriate for a subset of fibroblast cells or cancer cells such as NIH-3T3 and MDA-MAB 231 cells. Cells migrated toward chemoattractants were measured by crystal violet, a nucleic dye, and quantified by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, and chemokinesis.
The Millipore QCM 5 µm Chemotaxis Assay 24-well-colorimetric provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
• QCM 8µm 24-well Chemotaxis Cell Migration Assays (ECM508, 509)
• QCM 5µm 24-well Chemotaxis Cell Migration Assay- Fluorometric (ECM507)
• QCM 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
• QCM 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
• QCM 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
• QCM 96-well ECMatrix Cell Invasion Assay (ECM555)
• QCM 96-well Collagen-based Cell Invasion Assay (ECM556)
アプリケーション
The Millipore 5 µm QCM Chemotaxis Assay 24-well-colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle. The 5 µm pore size of this assay′s Boyden chambers is appropriate for studying a subset of fibroblast or cancer cell migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples.
The Millipore 5 µm QCM Chemotaxis Assay 24-well-colorimetric is intended for research use only; not for diagnostic applications.
The Millipore 5 µm QCM Chemotaxis Assay 24-well-colorimetric is intended for research use only; not for diagnostic applications.
包装
24 wells
構成
Sterile 5 µm 24-well Cell Migration Plate Assembly: (Part No. 2005708) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
Cell Stain: (Part No. 90144) one 20 ml.
Extraction buffer: (Part No. 90145) one 20ml.
Cotton Swaps: (Part No. 10202) 50 each.
Forceps: (Part No. 10203) One each.
Cell Stain: (Part No. 90144) one 20 ml.
Extraction buffer: (Part No. 90145) one 20ml.
Cotton Swaps: (Part No. 10202) 50 each.
Forceps: (Part No. 10203) One each.
保管および安定性
The 5 µm 24-well Cell Migration Plate and forceps are stored at room temperature. Cell stain and extraction buffer can be store at 2° to 8°C up to the expiration date. Do not freeze any components.
法的情報
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Flam. Liq. 2
保管分類コード
3 - Flammable liquids
引火点(°F)
53.6 °F
引火点(℃)
12 °C
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
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Jan Code
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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