CC077

Human Collagen Type V

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• 別名: Collagen Type V
• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

特性

• product name: Human Collagen Type V,
• 由来生物: human
• 品質水準: 100
• アッセイ: 95% (SDS-PAGE)
• 形状: liquid
• メーカー／製品名: Chemicon^®
• 濃度: 1 mg/mL
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.5% non-collagen proteins; <1% collagen type III; <2% collagen type I; <2% collagen type IV
• 入力: sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s); sample type epithelial cells; sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type: human embryonic stem cell(s)
• NCBIアクセッション番号: NM_000093.3
• UniProtアクセッション番号: P20908
• 輸送温度: dry ice
• 保管温度: −20°C
• 遺伝子情報: human ... COL5A1(1289) , COL5A2(1290)

詳細

詳細

Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen. It is minor collagen present in the extracellular matrix.

アプリケーション

Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR). It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.

生物化学的／生理学的作用

Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.

物理的形状

Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.

調製ノート

Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.

その他情報

Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg and HIV antibodies.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable