605190-M

Thrombin, Human Plasma

• 別名: Thrombin, Human Plasma
• CAS番号: 9002-04-4
• MDL番号: MFCD00007020
• UNSPSCコード: 12352202
• NACRES: NA.51

特性

• 由来生物: human plasma
• 品質水準: 100
• 詳細: Merck USA index - 14, 9383
• フォーム: lyophilized
• 比活性: ≥1000 NIH units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 溶解性: water: 1 mg/mL; aqueous buffer: soluble
• 保管温度: −20°C

詳細

詳細

Thrombin, a sodium-activated type II enzyme, comprises two anion binding exosites, ABE-I and ABE-II.[1] This serine protease enzyme is synthesized from zymogen prothrombin (factor II) in the liver.[1][2]

アプリケーション

Thrombin, Human Plasma has been used:

• as a component of endothelial growth medium (EGM) media for the transplantation and reisolation of Kaposi′s sarcoma-associated herpesvirus-human endothelial cell line (KSHV-HuARLT) cells from mice[3]
• for the fabrication of fibrin gels[4]
• as a component of EGM media for viral copy number analysis of KSHV-HuARLT cells and matrigel implant[5]

生物化学的／生理学的作用

Thrombin cleaves and converts fibrinogen into fibrin. It then activates factors V, VIII, XI, and XIII. Thrombin stimulates platelet activation and stabilizes the fibrin polymers.[6][7] It elicits a vital role in the last stages of the blood coagulation cascade.[1]

警告

Toxicity: Harmful (C)

単位の定義

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

物理的形状

Lyophilized from 200 mM NaCl, 50 mM citrate buffer, 0.1% PEG-8000, pH 6.5. Contains BSA as a stabilizer.

調製ノート

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

再構成

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 2 months at -70°C.

アナリシスノート

Complete activation from homogeneous prothrombin by SDS-PAGE

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H334,H335
• 注意書き: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable