17-128
Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)
Alkaline/Acid Phosphatase Assay Kit is routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP.
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About This Item
UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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アプリケーション
Used to detect/quantify: Alkaline/Acid
包装
Kit capacity: 100 assays
構成
Malachite Green Solution A (Cat.# 20-105)
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
NiCl2, 40mM (Cat.# 20-178)
pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
96-well microtiter plate
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
NiCl2, 40mM (Cat.# 20-178)
pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
96-well microtiter plate
品質
routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
シグナルワード
Danger
危険有害性の分類
Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2
ターゲットの組織
Lungs
保管分類コード
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
17-128:
試験成績書（COA）
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Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.
Ekman, P and Jager, O
Analytical biochemistry, 214, 138-141 (1993)
An investigation of the substrate specificity of protein phosphatase 2C using synthetic peptide substrates; comparison with protein phosphatase 2A
Donella Deana, A., et al
Biochimica et Biophysica Acta, 1051, 199-202 (1990)
Nathan J Castro et al.
Tissue engineering. Part A, 22(13-14), 940-948 (2016-06-15)
Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address
P P Van Veldhoven et al.
Analytical biochemistry, 161(1), 45-48 (1987-02-15)
A procedure, based on the complex formation of malachite green with phosphomolybdate under acidic conditions, to measure inorganic orthophosphate in the nanomolar range is described. The addition of polyvinyl alcohol is required to stabilize the dye-phosphomolybdate complex. The advantages of
A Donella-Deana et al.
Biochimica et biophysica acta, 1094(1), 130-133 (1991-08-13)
The four main classes of protein phosphatases (PP-1, 2A, 2B and 2C), although differing in their ability to dephosphorylate phosphopeptide substrates, invariably display a marked preference toward phosphothreonyl peptides over their phosphoseryl counterparts. Conversely, all the acidic and alkaline phosphatases
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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