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Merck
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主要文書

安全性情報

TMS-010

Sigma-Aldrich

Alcian-Blue Staining Solution

別名:

Alcian blue 8G, Alcian blue PAS stain, Alcian blue stain

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About This Item

UNSPSCコード:
12352207
NACRES:
NA.75
Pricing and Inventory is not available. Please check the Merck website for more information.

テクニック

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

品質水準

100

関連するカテゴリー

詳細

Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.

アプリケーション

Research Category
幹細胞研究
Research Sub Category
オステオバイオロジー

関節炎

品質

Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.

物理的形状

Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.

保管および安定性

Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ピクトグラム

FlameCorrosion
GHS02,GHS05

シグナルワード

Danger

危険有害性情報

H226,H314

危険有害性の分類

Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

保管分類コード

3 - Flammable liquids

WGK

WGK 2

引火点(°F)

102.2 °F - closed cup

引火点(℃)

39 °C - closed cup

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

消防法

第4類:引火性液体
第二石油類
危険等級III
非水溶性液体

労働安全衛生法名称等を表示すべき危険物及び有害物

名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

TMS-010-C:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

資料

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

プロトコル

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

質問

1–4/4 質問  

  1. What is the composition of the differentiation for TMS 010 C procedure?

    1 回答

    1. Product TMS-010 is a standard pH 2.5 solution of Alcian Blue that stains a number of substances, including chondrocytes, at this pH. The provided procedure is similar but not identical to the standard histology stain procedure found in most histology textbooks.

      役に立ちましたか？

  2. What is the concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product?

    1 回答

    1. The concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product is not provided. However, it is a classical textbook formulation typically consisting of 1% Alcian Blue in 3% glacial acetic acid.

      役に立ちましたか？

  3. Is there a procedure available for using TMS-010, and in this context, would you anticipate the alcian blue to be present in the nuclei, cytoplasm, or the entire cell?

    1 回答

    1. Depending on the preparation of Alcian Blue, the dye can be used to identify various substances. TMS-010 seems to have been formulated to stain chondrocytes, the cells that produce collagen. It's expected that the staining should be primarily in the cytoplasm. However, if the staining intensity is high, it might appear that the entire cell is stained.

      役に立ちましたか？

  4. Can you provide a protocol for a colorimetric assay that uses Alcian Blue to compare sulfated proteoglycan content in adherent cancer cell lines?

    1 回答

    1. The Alcian-Blue Staining Solution (cat. no. TMS-010-C) is a cell culture product from the cell culture portfolio, intended for cell-based assays, cell differentiation, and stem cell culture. For example, to identify chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells (MSCs).

      This product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.

      The Certificate of Analysis does not include any usage instructions and there is no separate product data sheet or user guide.

      The following paper may be helpful for the staining of stem cells:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432525/

      役に立ちましたか？

レビュー

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