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抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
アッセイ
>95%
フォーム
solution
包装
pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))
メーカー／製品名
Avanti Research™ - A Croda Brand 330002S
濃度
0.2 mg/mL (330002S-25ug)
輸送温度
dry ice
保管温度
−70°C
詳細
Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
アプリケーション
E06 mAb-biotinylated has been used in immunodetection.[1] It may be used in immunohistochemistry,[2] western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).
生物化学的／生理学的作用
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL),[3] oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys,[2] mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.
包装
0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)
法的情報
Avanti Research is a trademark of Avanti Polar Lipids, LLC
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
330002S-VAR:
330002S-25UG:
330002S-BULK:
最新バージョンのいずれかを選択してください：
Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma.
Palinski W, et al.
The Journal of Clinical Investigation, 98(3), 800-814 (1996)
Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells
Coliva G, et al.
Redox Biology, 23, 101123-101123 (2019)
Increased plasma oxidized phospholipid: apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression
Tsimikas S, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 27(1), 175-181 (2007)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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