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53347-U

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Ascentis® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 3 cm × 4.6 mm, HPLC Column

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1 EA
303,80 €

303,80 €

Prezzo di listino434,00 €Risparmia il 30%
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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1 EA
303,80 €

About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

303,80 €

Prezzo di listino434,00 €Risparmia il 30%
Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Nome del prodotto

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 3 cm × 4.6 mm

Materiali

stainless steel column

Livello qualitativo

100

agenzia

suitable for USP L11

Nome Commerciale

Ascentis®

Caratteristiche

endcapped

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Parametri

60 °C temp. range
600 bar max. pressure (9000 psi)

tecniche

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

Lungh. × D.I.

3 cm × 4.6 mm

Area superficiale

135 m2/g

Impurezze

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Gruppo funzionale matrice

phenylhexyl phase

Dimensione particelle

2.7 μm

Dimensione pori

90 Å

Intervallo di pH

2-9

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Descrizione generale

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
USRZ001318
USRZ001315
USRZ001294
USRZ001292
USRZ001291

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
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Articoli

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Domande

1–10 di 10 domande  

  1. What is the pressure rating for Ascentis® Express Phenyl-Hexyl HPLC Columns?

    1 risposta

    1. This Ascentis Express column is stable to operating pressures up to 600 bar (9000 psi).

      Utile?

  2. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 risposta

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Utile?

  3. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 risposta

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Utile?

  4. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  5. Can I use Ascentis Express on a UHPLC system?

    1 risposta

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Utile?

  6. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 risposta

    1. Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)

      Utile?

  7. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 risposta

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Utile?

  8. Can I use Ascentis Express on any type of HPLC system?

    1 risposta

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Utile?

  9. Can a 100% aqueous mobile phase be used with the Phenyl-Hexyl HPLC column?

    1 risposta

    1. The phenyl-hexyl phase is 100% aqueous compatible.

      Utile?

  10. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 risposta

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Utile?

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